... be considered as the “base-line 8 910 11 12 Proteomic Applications in Biology 220 a. Alkaline-inducible proteins Code YL Swiss-Prot acc. # Function Protein cell localization Gene ... Vybornaya, T.V.; Larina, A.S.; Matsui, K.; Fukui, K. & Sineoky, S.P. (2010). Production of succinic acid at low Proteomic Applications in Biology 224 pH by a recombinant strain of the aerobic ... 3, 4 and 5) included the instant resuspension of the yeast cells in a hot lysis buffer containing urea, reducing agent, Triton X-100 and ampholytes. The second included a preliminary ultrasonic...