... diluted into DNA extracted from a lymphoblastoid B-cell line, in order to obtain decreasing concentration of tumoral DNA.Straight line: regression line for KRECs; dotted-line: regression line for ... obtained by extrapolating the respective samplequantities from the standard curve obtained by serial dilu-tions (106,105,104,103,102, and 10) of a linearized plas-mid DNA, containing ... diseasestage(BinetstageBandC),andbecausetheyidentifiedTreg as CD4+CD25highcells while, according to Liuet al [10], we more finely targeted this subpopulation by includinginTregsubsetonlyCD4+CD25int/high-CD127low/-lymphocytes....