... with the activity values in the presence of EDTA (86 . 18 ±13. 78 UÆmg)1) or in the absence of chelators (90.94 ±7 .85 UÆmg)1). These results suggest that the presence of calcium is important for ... for the optimal activity of BsArb43B.Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were per-formed to determine the Tm of the protein in the ... parametersa, b, c (A˚) 51.9, 57.9, 85 .6 51 .8, 57.4, 85 .5 51.9, 57.6, 86 .2a, b, c (°) 96.2, 91 .8, 117.3 82 .1, 88 .2, 63.6 82 .3, 87 .9, 63.6Number of unique hkla133 640 76 283 67 116Completeness (%)a94.9...