... for the optimal activity of BsArb43B.Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were per-formed to determine the Tm of the protein in the ... c (A˚) 51.9, 57.9, 85 .6 51.8, 57.4, 85.5 51.9, 57 .6, 86. 2a, b, c (°) 96. 2, 91.8, 117.3 82.1, 88.2, 63 .6 82.3, 87.9, 63 .6 Number of unique hkla133 64 0 76 283 67 1 16 Completeness (%)a94.9 ... gave the following rmsd val-ues: 1.50 A˚ for 192 Ca pairs, 1 .66 A˚ for 189 Ca pairs,1 .68 A˚ for 211 Ca pairs and 1 .66 A˚ for 189 Ca pairs,respectively (Fig. 2). These values show that the...