... protein in crude membrane fractions with anti-PCaP1. Lanes 1 and 6, A. thaliana; lanes 2 and 7, Raphanus sativus; lanes 3 and 8, Brassica rapa;lanes 4 and 9, B. rapa var. glabra; lanes 5 and ... protein.As shown in Fig. 2A, a highly purified preparation ofPCaP1 without any tag was obtained. The protein wasanalysed by SDS-PAGE and immunoblotting with ananti-PCaP1 IgG to obtain a calibration ... medium at 22 °Cin the dark. Other plants [Raphanus sativus (radish), Bras-sica rapa (turnip), Brassica rapa L. var. glabra Regel (Chi-nese cabbage) and Brassica oleracea var. italica (broccoli)]were...