... per-formed all the experiments and drafted the manuscript.SHK participated in the design of the study and conceived of the study. RBHI participated in the design of the study and conceived of ... 65°C for 5 minutes, 42°C for 2minutes, 42°C and 70°C for 50 minutes and 15 minutes.Polymerase chain reaction was carried out using theTakara thermal cycler (Takara Bio Inc. Japan). Six-μl of ... (Figure 3A, B, C and Figure 3G, H, I) and PLGA (Figure 3D, E, F and Figure 3J, K, L) were clearly visible in term of overall cartilaginous tissue formation, cells organization and ECM distribu-tion....