... developing new ways of preventing and
managing allergies and asthma. In the long term, the
research network aims also to decrease the socioeco-
nomic burde n of allergy and asthma in Europe. In close
association ... contributions and an increase in both
the amount of research and networks was found.
Introduction
Allergic diseases are complex inflammatory conditions
w...
... cells
in vitro assay.
Methods
Cell lines
K562 cells (American Type Culture Collection) were
maintained in DMEM medium. Colorectal carcinoma cell
lines (COLP-2 and COLM-6) were maintained in TIL
Media ... induction of Treg in vivo
and in vitro in a murine model [52].
Moreover, the blockade of TGF-β reduces Treg induction
by the fusion cell vaccine and enhances antitum...
... strain and the vaccinal strain of MYXV, indicating that SG33 could be used for new bovine
vaccination strategies.
Background
Until now, most of the ruminant vaccines use attenuated
strains of ... between
bovine cells (cell lines and PBMC) and MYXV wild type
(T1) strain or vaccinal (SG33) strain. In bovine cell lines,
serial viral passages analysis and infection with both T1...
... membrane potential is
represented as ratio of fluorescence at 590 and 535 nm measured by JC-1 staining at 48 and 72 hr after infection, and
expressed as percentage decline in infected cells grown in ... analyzed using fluorogenic substrate
at day 2 after infection in WNV-infected and mock-infected cells. The data are expressed as percentage increase of caspase
activity...
... grown in vitro under three-dimensional condi-
tions using a mixture of collagen and fibroblasts [32,44].
Interaction of a2andb1 integrins on CRC-specific
T cells with collagen and the presence of ... cells), fixed in buffered formalin and embedded in paraffin. a, b: Staining with H&E. c, d: Specific
brown staining of apoptotic cells by anti-caspase 3 Ab. e, f: In s...
... 5 min of incubation with 0.4%
Trypan blue, the percentages of stained cells (indicative of
nonviable cells) versus stain-excluding cells were counted
in a hemocytometer. Then the percentage of ... for 3
min, and placed on ice until the addition of the remaining
reaction components. The reaction was incubated at 42°C
for 90 min, and terminated by heat inactivation at 70°C
for 1...
... dissec-
tion of the folding mechanism of the a subunit of tryp-
tophan synthase: an amino-terminal autonomous
folding unit controls several rate-limiting steps in the
folding of a single domain protein. ... display),
increasing the likelihood of correct folding in a redu-
cing environment while minimizing the risk of proteo-
lytic degradation. The association of plasmid and...
... to determine the role, if any, of chlo-
roquine in the prophylaxis and treatment against
influenza infection. It would also be interesting to deter-
mine the activity of chloroquine against the ... decreasing concentration of chloroquine
and this is similarly reflected in the decrease in Ct value of
the real time RT-PCR of their respective supernatant. The
IC50 and IC90...
... obtained after investigating neuroserpin
effects using an in vitro model of brain ischemia [40].
The information reported here regarding b iomarkers
might be relevant to evaluate the utility of ... closed.
Theabsolutedifferencebetweenbasaland24hours
neuroserpin levels was defined as neuroserpin decrease.
All determinations were carried out in a laboratory
blind to the clinical out...
... low frequency.
One of the most interesting findings in this study is the
wide variability in distribution of sexual risk in the popu-
lation. The number of individuals engaging in risk behav-
iors, ... with HIV infection and
provide insight into the growing rate of drug resistance in
incident HIV infections in areas where ARVs has been
available for several decades.
In...