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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx

báo cáo hóa học:

báo cáo hóa học:" Aberrant expression and potency as a cancer immunotherapy target of alpha-methylacyl-coenzyme A racemase in prostate cancer" pptx

... the design of the study and performed the evaluation of the data. TT helped to draft the manuscript. YH contributed to the HLA-A24-binding assay and CTL induction from PBMCs. HK, ES and NM contributed ... indicating the adequate qualification of this assay. The HLA-A24level on the cell surface of T2-A*2402 cells was up-regu-lated in the presence of AMACR1, AMACR2 and AMACR3peptides, but not in the ... slightlylower than those of previous reports. On the other hand,its expression was very low in benign prostate glands,which showed only focal and weak staining [6]. The func-tion of AMACR in prostate...
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báo cáo hóa học:

báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

... emerged from Finland, suggestingthat the lack of CCR5 does not protect from MS, but ratherit may predispose to the chronic course of the disease [69].This would further imply that in view of the ... purposes)macrophages and resident microglia to sites of CNS inflammation. Detailed knowledge of expression patterns is crucialfor the understanding of therapeutic modulation and the validation of CCR1, CCR2 and ... immu-noneutralization of CCL3 [44], DNA vaccination [45], orgenomic deletion of the CCR1 gene [22], reduces clinicaldisease. Taken together, the results of the present study and from previous ones on the role of...
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báo cáo hóa học:

báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx

... following the development of drug resistance. A. Heat map showing the expression of all the significant genes analyzed using the Illumina bead array (845 genes). Changes in gene expression for the ... to the parental OV90 line. B. Multi-dimensional scaling plot indicating the cell lines used for the gene expression profiling analysis. Each of the two different resistant clones obtained from ... experiments design and analysis. WHW performed the microarray experiments. YZ analyzed the microarray data. PJM conceived the study, oversaw the experiments, analyzed the data, and drafted the manuscript....
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... of several pathologies and because of the potential use of the toxins as biological weapons. Alteration of their MHC and TCR binding capacity by site directedmutagenesis could be useful in the ... two bands of protein were observed in SDS/PAGE (Fig. 1A). The weaker band has an apparent molecular mass in agree-ment with the theoretical value calculated from the aminoacid sequence; the ... on the opposite f ace of the molecule that is Zn2+dependent [14,15]. The proinflamatory and procytotoxic properties of SAgsare r esponsible for the increased interest in these moleculesin the...
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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx

Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx

... regulate the biosynthesis of the lipopeptides. The experiments performed in the present study showedthat this AAT can form Glu and Asp, and the forma-tion of Glu and Asp is used to synthesize Gln and Asn, ... determine the exact role of three residues in AATB3. The loss of the activity from the mutations together with the multiplealignment analysis indicated that the Asp232 residue of AATB3 enhances the ... cloned from the B. subtilis B3 strain and analyzed phylogenetically.We also describe the expression in E. coli and charac-terization of the recombinant enzyme by determining the optimum pH and...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt

Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt

... levels in the m edial striatum of a ny of the w ild-type and HD mice examined. The optical density of the hybridization signal in the lateral striatum, corrected bysubtracting the signal in the m ... length of the CAG repeat and relative expression of the human HD transgene in the twostrains of mice and that mutant huntingtin differentiallyaffected CB1 mRNA levels in specific types of neurons.Mus ... defining the CB1 promoter, w edetermined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparingthe...
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Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx

Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx

... way except for the presence of the linker and the family-2 CBM at the Cterminus of XEG74. The molecular masses of Cel9A-68(CD + family3c CBM) and Cel48ACD, both cloned with the Cel6A signal ... glucose standard curve and the nmoles of XGOs produced was determined for both the PAHBAH and the bicinchoninic acid reducing sugarmethods. For the PAHBAH method, the nmol of XGOs ¼ nmol of glucose ... Xeg74. This mayreflect the presence of small amounts of polysaccharidesother than XG in the extract and/ or variability in the structure of tomato XG. For example, the AXG of solanaceous plants,...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot

Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot

... volume. Nitrite released from the reaction wasdetermined by the method of Little [28]. One unit of NaoAis defined as the amount of enzyme required to catalyze the formation of 1 lmol nitriteÆmin)1. ... to the experimentto identify whether the protein encoded by the completeDNA fragment can catalyze the oxidation of nitroalkanes.In this paper, we describe the cloning and characterization of a ... standard.Properties of NaoA The pI of NaoA is about 5.2 according to the standard plotbetween the protein’s pI and its migration distance (cm) inIEF. The optimal pH and temperature of purified NaoAwere...
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báo cáo hóa học:

báo cáo hóa học:"On quotients and differences of hypergeometric functions" pptx

... −xy), and the proof of the first part of Theorem 3.6 is complete. The proof of the second part is similar. Note that the condition c ∈ (1/2, ∞), d ≥c/(2c −1) of Lemma 2.11 is equivalent to the ... parts (1) and (4) of Lemma 2.11 give R −B + 1 < 0 and B − 1 < 0.Because the right-hand inequality is a consequence of Theorem 3.6, part (2), the proofis complete. 4.9. Proof of Theorem ... condition 1/c + 1/d ≤ 2 of Theorem 3.6. 4.8. Proof of Theorem 3.7.(1) The left-hand side of this inequality is a direct consequence of part (1) of Theorem3.6.Next, from Lemma 2.11, part (3)...
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báo cáo hóa học:

báo cáo hóa học:" Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels" docx

... higher than 3.5 kg. The expression of specific endothelial cell markers (von Willebrand factor, CD31, and the binding and internalization of acetylated low-density lipoprotein) and the smooth muscle ... cellcultures and carried out the molecular and cellular analy-sis. CGV and JLF informed the parents about the objec-tives of the research project, did the anthropometricmeasurements at birth and obtained ... determining the percent-age of cells expressing Ki67 (see below) from the totalnumber of cells.Cellular markers The expression of von Willebrand (vW) factor, CD31(platelet endothelial cell...
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