Báo cáo toán học: "Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168" ppt
Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... allowing the interaction of Antarctic b-tubulin isotypes with CCT in the adverseenergetic conditions of the Antarctic habitat. The role of these amino acid substitutions in the folding process of E. ... analysis of the interaction between CCT and b-tubulin by cryoelec-tron microscopy allowed the identification of three regionsfrom the N-terminal, and five from the C-terminal domains of b-tubulin, involved ... unlabeledtubulins are indicated on the top of each lane and the arrow indicates the position of the b-tubulin/CCT complex. (B) The amounts of the tubulin bound to CCT were quantitated by the use of a...
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Báo cáo khoa học: Heterologous expression of a serine carboxypeptidase-like acyltransferase and characterization of the kinetic mechanism potx
... predicted to bemainly involved in l-malate recognition and bindingresulted in strong reduction of enzyme activity. The inhibition of SMT catalysis by d-malate reveals the positioning of the reactive ... (Fig. 3). The in vitro kinetics of SMT was examined byassaying the conversion of 1-O-sinapoyl-b-glucose(sinapoylglucose = singlc) to 1-O-sinapoyl-l-malateHeterologous expression and kinetic mechanism ... attaching restric-tion sites for HindIII and XbaI to the 5¢- and 3¢-ends of the product. By cloning as HindIII-XbaI fragments into the expression vectors pYES2 (Invitrogen) or pDIONYSOS, the PCR...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... ACMSD of the region surrounding the metal active site and the Arg47 likely involved in substrate binding.Discussion In this study, the expression of the human ACMSDtranscript coding for the active ... in the brain of a murine model of the syndrome[18,19]. Interestingly, picolinate is reportedly able toprevent the neurotoxic effects of quinolinate in the ratcentral nervous system, suggesting ... electrophoresis of the PCR product indicated the presence of two distinct bands between 1000 and 1100 bp of roughly the same extent (not shown). Clo-ning and nucleotide sequencing of these two productsindicated...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... result of the lostCa2+⁄ CaM-binding site of the IQ domain, but the missing PH1- and coiled-coil domains may also change the subcellular localization of this version of Ras-GRF1. These domains ... (F340) increases, whereas the fluorescence intensity at 380 nm (F380) decreases upon the binding of Ca2+. The change in the ratio of F340 ⁄ F380 determined the change in Ca2+concentrationinside ... physiological role of the guanine nucleotide exchange activity of the truncatedforms is not known as they are missing the Ca2+⁄CaM-binding IQ domain that is involved in the activa-tion of Ras-GRF1.Stimulation...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... hypothetical. Although most cystatins inhi-biting cathepsin L also inhibit cathepsin S, an inhibitoryactivity of cystatin F against cathepsin S has not yet beenreported. The expression of the ... of the cystatin F gene and promoter. The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustratedschematically at the top. In the upstream region of the CST7 gene, the ... by their affinity to Cm-papain, stronglyindicating that the carbohydrate side chains do not affect the enzyme-binding properties of cystatin F and should, thus, not affect the inhibition of...
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Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the C-terminal ends of a helices providing the conforma-tional ... functions in many plants beyond the scope of pigmentation and ultraviolet screening, e.g. in reproduction [1], in the defense against microbial pathogens and insects or in auxin transport [2], and ... determined according to Lowry[39] following the precipitation with trichloroacetic acid in the presence of deoxycholate [40] and using bovine serumalbumin as a standard.Mass spectrometryThe...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecularstudies of the interaction of SAgs with their s pecifics ligandswill not only advance understanding of the physiologicalmechanisms of these molecules, but may lead to the development ... been shown to b e the TCR binding site in other SAgs. Point mutation of Cys26 to Ser prevented dimerformation and allowed TCR interaction studies.Initial binding experiments using a biosensor ... reduction and alkylation, and mutation of the Cys implicated in the intermolecular disulfide b ridge. Analysis of t he three-dimen-sional structure of SSA [31] to determine which of the remaining three...
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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... protein ligand tethering the co-enzyme in a productive mode within the active site and stabi-lizes the protonated N(1) of the co-enzyme tostrengthen the electron-withdrawing capacity of the co-enzyme ... function instead in subgroup Ib [18]. Arg386 of the small domainbinding the a-COO) of the substrate plays a key role in the activity of the enzyme [19,20]. The functions of the above-mentioned ... industrial applications, such as in the synthesis of l-tyrosine, l-phenylalanine and l-homophenylalanine. A detailed characterization of the role of B. subtilis B3 AAT and its structure are in progress.Materials...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... describing the genestructure of CB1 and i ts pattern of expression in transgenicmice will provide the information necessary to determinehow mutant huntingtin alters the expression of this ... in HD mouse brain. T his supports the hypothesis that the rate of transcription of CB1 in the striatum of symptomatic R6/2mice is similar to the rate of transcription i n regions of the brain ... length of the CAG repeat and relative expression of the human HD transgene in the twostrains of mice and that mutant huntingtin differentiallyaffected CB1 mRNA levels in specific types of neurons.Mus...
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Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx
... Da), indicatingthat the signal peptide was being cut in different places,resulting in the addition of up to 17 extra amino acids at the N terminus of the protein. It is not clear why the Xeg74CD ... [11,22] using this signal sequence and it has a convenient NotIsiteatitsC terminus, which allows cloning in- frame with alanine as the first amino acid of the mature protein. An alignment of the amino ... bacterial wall and which coalesce into bundles of highly crystalline microfibrils [25]. When G. xylinus wasgrown in the presence of glucose and tamXG, 38% of the added XG was incorporated into the cellulose...
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Từ khóa: báo cáo khoa họcBáo cáo y họcbáo cáo khoa học toán họccác báo cáo toán học haybáo cáo toán học hayNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ