... DNA AMPLIFIERMIR-D40 (Sanyo, Osaka, Japan). To amplify the DNA frag-ments containing a complete x-5 gliadin gene, oligonucleo-tides, 5 ¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢and 5¢-CGTTACATTATGCTCCATTGACTAACAACGATG-3¢, ... kit and the ABI 3100 DNA sequencer(Applied Biosystems, Foster City, CA, USA).Expression and purification of recombinantproteinSense (5¢-ATTTCATATGCAACAACAATTCCCCCAGCAACAATCA-3¢) and antisense ... Total genomic DNA was isolated from 0.1 g frozenleaves by the Isoplant DNA extraction Kit (Takara Bio Inc.,Shiga, Japan). PCR was performed using KOD DNA polym-erase (Toyobo, Osaka, Japan) and...