... anti-inflammatory/anti-oxidant functions of AICAR, we tested its inhibitorypotential in blocking the expression of pro-inflammatory cytokines and iNOS, expression of COX-2, generation of ROS, and ... by blocking the expression of proinflammatory cytokine, iNOS, COX-2 and MnSOD genes, and by inhibition of ROS generation and depletion of glutathione in astroglial cells. AICAR also inhibited ... (1–42) in concentrations as indicated. The corresponding reverse peptide (40–1) in lane 3 and 9 served as a positive control in this assay. The production of NO (top) and expression of iNOS,...