... ERM proteins and NF2 tumorsuppressor: the Yin and Yang of cortical actin organization and cellgrowth signaling. Curr Opin Cell Biol 2002, 14(1):104-109.5. Mielgo A, Brondani V, Landmann L, Glaser-Ruhm ... were annealed and inserted into the BamH I and Hind III sites of pSilencer2.1-U6 and transformed into JM109 competent cells(Promega,Madison,WI,USA).Positivecloneswereidentified and verified ... inia,USA) was grown in DMEM (GIBCO, Grand Island,New Yolk, USA) supplemented wit h 10% fetal calfserum (FCS) and 1% L-glutamine (Invitrogen, Karls ruhe,Germany) and maintained at 37°C in 5% CO2....