báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... bHLH
region, including His57 and Arg65, is responsible for t he
E-box binding.
Determination of the region in BMAL1 for binding
to DEC1
To identify the region in BMAL1 required for the binding to
DEC1, ... C-terminal region, including the PAS-B
domain of BMAL1, is required for the binding to DEC1,
Fig. 4. Identification of the domain of D EC1
that interacts with BMAL...
... [14,20]. Binding of CCE1 results in the
complete unpairing of the four base-pairs in the centre of
the junctions with a lesser disruption of the next base-pairs
[19], which is reminiscent of the structure ... conformation
of the junction, then the deletion of this motif would have an
even more serious effect on Ydc2 binding in the presence of
divalent c...
... interestingly, in contrast with the
lack of other components of the peroxisomal protein
import machinery, the deficiency of Pex4p does not
interfere with the assembly of the components of
the importomer. ... a
control. The corresponding solubilisates
(right panel) were analyzed for the presence
of Pex1p and Pex6p as indicated. The defici-
ency of either prote...
... glycosaminoglycans [37].
As the myc-his tag of the protein was present at the
C-terminus of the receptor which is located on the inside
of the cell, we then determined if signal transduction by
the ... plasmid. The
resulting XhoI/ApaI fragment was cloned into the corres-
ponding sites in the polylinker region of the pcDNA3.1-
myc-His expression vector (Invi...
... initiate a bioinformatic anal-
ysis of plant b-glucosidases to obtain insights into the
evolution of the molecular sites involved in the two
modes of aglycone binding.
The amino acid sequence of Zm-p60.1 ... aglycones in Zm-p60.1. Interest-
ingly, both lysine residues and the leucine residue are
conserved in all five enzymes of the group, and the
alanine resid...
... using the
programme Microsoft Excel 2000. The curve for the determination of
the K
d
forATPwasobtainedusingtheprogram
KALEIDA GRAPH
3.0.
Fig. 5. ATP-binding capacity of PfPDO. Cross-linking of ... reduces insulin disulfide in the presence of dithio-
threitol at 30 °C. The a nalysis w as performed in the
presence of increasing concentrations of the pure proteins,...
... ostly in
eukaryotic organisms, in which the intermediate complex is
first formed by electr ostatic attractions and the further
reorientation mainly involves hydrophobic interactions.
The kinetics of ... oli. The construction of the synthetic
gene, e xpression c onditions, purification protocol and
characterization of the recombinant protein will be pub-
lished elsewher...
... plots.
Determination of the metal contents of the Qor proteins,
and detection of the nucleotide moiety of the
molybdenum cofactor
The contents of molybdenum and iron were determined by
inductively ... from quinoline [1,2]. Besides quino-
line, some quinoline derivatives and the benzodiazines
quinazoline and quinoxaline are accepted as substrates [1,3].
Like other...
... against the
nuclear receptor FXR (lanes 3 and 4), or against retinoid
X receptor (RXR) (lanes 5 and 6) or a combination of both
(lanes 7 and 8) resulted in a reduction of signal intensities of
the ... effects [27]. Mutation of the IR-1 element
within the minimal promoter resulted in a loss of CDCA
reporter stimulation, indicating the functional importance
of...