... in Single Living Cells. Nat. Methods 4( 5), 41 3 41 9. Ozawa, T., Nogami, S., Sato, M., Ohya, Y., & Umezawa, Y. (2000). A fluorescent indicator for detecting protein-protein interactions in ... thrombin, AGP and HSA. Strong binding occurred in the case of RS-melagratan-trombin interaction. The other enantiomer did not interact at all with the protein (Sandblad et al., 2009) 4. 3 Ion ... fluorescence detection of Ins(1,3 ,4, 5)P 4 by the excitation of each distinct absorption band, and retained ligand affinity and selectivity of the original PH domain. Fig. 1. Schematic illustration...