báo cáo hóa học:" Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture" ppt
... Helsinki, Finland
Email: Angelina Plyusnina - anguelina.pljusnina@helsinki.fi; Alexander Plyusnin* - alexander.plyusnin@helsinki.fi
* Corresponding author
Abstract
Tula hantavirus carrying recombinant ... parental virus: analysis of consecutive
passages in a cell culture
Angelina Plyusnina and Alexander Plyusnin*
Address: Haartman Institute, Department of Virology,...
... in this paper show that the recTULV
presents no real match to the original cell adapted variant
and that the lower fitness of the recombinant virus can not
be increased by pre-passaging in cell ... weaker due to the fact that two non-canonical G:U
base pairs presented in the plus-sense RNA occur as non-
pairing C /A bases in the minus-sense RNA. Interestin...
... Public Health and Neuroscience, University of Pavia, Pavia,
Italy
Full list of author information is available at the end of the article
Tonini et al . Journal of Occupational Medicine and Toxicology ... and interpreted the data. FM collected,
analyzed and interpreted the data. CF collected, analyzed and interpreted
the data, performed the statistical analysis. FS parti...
... F) is the relative frequency of codebook F in
object instance l.
We have to minimize intraclass variations. As mentioned,
one of the main causes of large intraclass variation is
surface markings, ... comparing visual appearance.
In Figure 10, wheels in the car codebook and in airplane
codebook have a similar appearance. At the same time,
each category also has a...
... but seconding them, as in the case of
additional provincial clinical care specialists, additional
capacity remains external and limited to the period of GHI
funding available. The more Zambia's ... of their clinic, imposing a further strain on the
day -to- day running of the ART programme.
Mentoring and the secondment of GHI staff to the public
sector...
... icosahedron. The P domain is divided into sub-
domains P1 and P2 that participate in dimeric contacts
that increase the stability of the capsid. The P2 domain is
an insertion in the P1 domain and ... 5'- GGG GAT ATC CAC CAT GGA GAC AGA CAC ACT CCT GCT AT -3'
VK2-F 5'- GGG GAT ATC CAC CAT GGA TTT TCA AGT GCA GAT TTT CAG -3'
VK3-F 5'- GGG GAT ATC CAC...
... made
contribution in data analysis; CG made contribution to the analysis and
interpretation of the data, in critical revision of the manuscript and in
acquisition of funding. PDB conceived and designed ... the study, analysed and
interpreted the data and drafted the manuscript. All authors read and
approved the final manuscript.
Authors’ information
LP’s present...
... N-terminal His-tag to allow
a functional expression of the 2,5-DKCMO in E. coli
and easy protein purification by affinity chromatography.
The N-terminal tag was favored compared to the
C-terminal tag, ... (DBU, Osnabrück,
Germany, Grant No. AZ13234) for financial support and Christin Peters and
Ina Menyes for assistance in the laboratory.
Competing interests
The authors...
... respectively.
The analysis of variance (ANOVA) tested using Fisher’s statistical analysis, was
used to verify the adequacy of the model. The results are shown in Table 3. The model F-
value of 99.32 ... activity of recombinant bromelain. An
increase in recombinant bromelain production was seen by increasing the L-arabinose
concentrations from 0.1−0.2 % (w/v...