... (DCI)DCI was performed after the original analysis. OSNAruns were repeated from discordant sample homoge-nates and afterwards RNA was isolated and subjectedtoqRT-PCRforCK19,CEA,andbeta-actin.Condi-tions ... Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F,Kato Y, Tsuda H, Ueda S, Sato K, Tamaki Y, Noguchi S, et al: One-stepnucleic acid amplification for intraoperative detection ... 4).Discordant case investigation (DCI) For all discordant samples additional OSNA runs as well as qRT-PCR for CK19 an d CEA were conducted fromthe remaining homogenate. However, since prolongedstorage...