... For EC and CY, reactions were initiated by addition of
0.1 mL of EC or CY to 1.0 mL of VM containing 10 lgof
GOU. For HP and M, reactions were initiated by addition
of0 .1gofHPorMto1.0mLofVMcontaining10lgof
GOU. ... a
membrane fraction (M) from cultures of 2BW-1 (see
Materials and methods). Enzymatic activity was determined
Fig. 6. GC and GC-MS analysis of TMS-derivatives...
... 9:57
http://www.translational-medicine.com/content/9/1/57
Page 2 of 12
RESEARC H Open Access
Detection and characterization of translational
research in cancer and cardiovascular medicine
David S Jones
1,2*
, Alberto Cambrosio
3
and Andrei Mogoutov
1,4
Abstract
Background: ... as the number of
links between those two nodes (i.e., the number of times
X and Y were co-cited)...
... and 215 nm, indicating that Rv1399c is
composed of 22% o f a-helices, 25% of b-strand a nd 39% o f
random coil.
The catalytic triad is located at the bottom of a small
pocket, 20 A
˚
length and ... 7.0).
Ó FEBS 2004 Biochemical characterization of Rv1399c from M. tuberculosis (Eur. J. Biochem. 271) 3955
Expression and characterization of the protein Rv1399c
from
Mycoba...
... similarity of the A- and
B-chains of the four isoforms, a pairwise alignment
was performed and the values of identity expressed in
Fig. 3. (Continued).
Characterization of four pulchellin isoforms ... M
r
values of the standards in thousands. Lanes 2–9, 5 lg of peak
P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec-
tively, in the presence (lanes 2–5) o...
... Buchert
1
and Kristiina Kruus
1
1 VTT Technical Research Centre of Finland, Espoo, Finland
2 Protein Chemistry Research Group and Core Facility, Institute of Biotechnology, University of Helsinki, ... Da). The identity of this peptide was
then confirmed by MALDI-TOF ⁄ TOF fragment ion
analysis, where partial sequences of this peptide were
obtained from the ladders of b-fragme...
... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D),
wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O). Panel M is the control ... 7A, D, I and L are diamidino-2phenylindole
hydrochloride (DAPI) stained cells showing the nuc-
leus, and Fig. 7C, F, K and N are the merged images
of A and B, D an...
... 10.0;
GAPEXT
,0.05;
GAPDIST
,8;
MAXDIV
,
40;
ENDGAPS
,off;
NOPGAPS
,off;
NOHGAPS
,off.Graphical
output of the bootstrap figure was produced by the program
TREEVIEW
.
Isolation of RNA and RT-PCR analysis
Total RNA was isolated from RTG-2 cells and ... RT-PCR, were obtained from the
Nikko Branch of the National Research Institute of
Aquaculture (Tochigi, Japan) and reared on a...
... properties. The cause of
these differences is not known.
K
m
and
k
cat
measurements of hevamine and mutants
Comparison of the steady-state kinetic parameters of
hevamine and the Tyr183Phe and Asp125Asn ... the
carbonyl oxygen atom of the N-acetyl group of the )1 sugar
residue has rotated away from the C1 atom of t hat residue.
The c ombined s tructural and k inetic d...
... a competitive mode of action.
Uptake of Bip-[
3
H]Pro by SKPT cells
After characterization of Bip-Pro as a very high-affin-
ity and enzymatically stable substrate of PEPT1 and
PEPT2, the compound ... recovered from
monolayers of both cell types intact to > 99% (HPLC
data not shown).
Interaction of Bip-Pro with PEPT1 and PEPT2
We next determined the interaction of Bip...
... amide and carbonyl of Cys140 form hydro-
gen bonds with the backbone carbonyl and amide of
Trp123, the side chain of which forms one wall of the
active site cavity and the replacement of which ... of
the vector by digestion with NcoI and PstI. This vector
backbone was excised from an agarose gel and purified. For
both standard and error-prone PCR amplification of
hMn...