... Tomaru Y, Katanozaka N, Nishida K, Shirai Y, Tarutani K, YamaguchiM, Nagasaki K: Isolation and characterization of two distincttypes of HcRNAV, a single-stranded RNA virus infecting the bivalve-killing ... lysateswere treated with RNase (Roche, Mississauga, Canada)and then extracted with a QIAamp Minelute Virus Spin Kit(Qiagen, Mississauga, Canada) according to the manufac-turer's instructions. ... drafted the manuscript.AL contributed to the design of the study, analyzed the data and helped prepare the manuscript. CS was involved in the conceptualization and design of the research and in manuscript...