... cells) , fixed in buffered formalin and embedded in paraffin. a, b: Staining with H&E. c, d: Specificbrown staining of apoptotic cells by anti-caspase 3 Ab. e, f: In situ end-labeling (ISEL); ... grown in vitro under three-dimensional condi-tions using a mixture of collagen and fibroblasts [32,44].Interaction of a2andb1 integrins on CRC-specificT cells with collagen and the presence of ... class I and IImolecules, FAS, ICAM-1, and various integrins.Expression of a2 and b1 integrins by the CRC cells mayfacilitate their binding to collagen [42]. CRC cells alsoexpress FAS ligand (<...