Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
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Research
Development of a real-time RT-PCR and Reverse Line probe
Hybridisation assay for the routine detection and genotyping of
Noroviruses in Ireland
John F Menton*, Karen Kearney and John G Morgan
Address: ... hospitals.
Results: A real-time RT-PCR assay and a Reverse Line Blot Hybridisation assay were developed
based on...
... therapeutic potential
and is a very promising candidate for ovarian cancer ther-
apy in humans.
Materials and methods
Cell culture
The human ovarian carcinoma cell lines (ES-2, SKOV-3,
TOV-112D and ... PO participated in design, coordination, and
data interpretation and drafted the manuscript. All
authors read and approved the final manuscript.
Acknowledgements
We th...
... tool
used for data analyses and reviewed statistical analyses. YG participated in the
assessment and selection of TaqMan PCR assays for studies in monkeys and
participated in the biomarker discovery ... securing and scheduling the resources that will be required upon hand-off
for assay validation. SJ, AW, SWA and KA performed the in vitro assays on mon-
key...
... software. Another 4 healthy mice were used as control. The total tumor area of each bladder was determined and
the mean of the total areas was calculated for each group. The Mean and SD of bladder ... participated in
design, interpretation of data and critically revised the manuscript. All
authors read and approved the final manuscript.
Competing interests
The...
... what kinds of caregiver measures were avail-
able in the literature. We conducted a literature search
(updated in October 2008) using the PubMed,
PsychINFO and AgeLine databases and using search ... correlations with a scale other than the hypothe-
sized scale were moved, and the multitrait scaling analysis
was run again. Items loading on more than one scale
(applying...
... maximum validity and reliability
of the results obtained, and constitutes a safe starting
point for examining the usefulness of the tool. On the
other hand, the tool includes the main evaluative
domains ... complete and rapid evaluation of patients with fibromyalgia.
The test intrinsically evaluates the emotional aspects: anxiety and depression, and their i...
... exercise,
analysed the validation data and contributed to the writ-
ing of the manuscript. JB designed and managed the valu-
ation survey, analysed and reported on the valuation data
and contributed ... interesting to note that in the
development of the EQ-5D the state of perfect health was
not valued and was assumed to be 1 [19]. This anchoring
meant that...
... design of the
study, collected the data and performed the statistical
analysis BD and VM: participated in the design of the
study, collected medical data and participated to the inter-
pretation of ... Fujisawa M, Ichikawa Y, Yoshiya K, Isotani S, Higuchi A, Nagano S,
Arakawa S, Hamami G, Matsumoto O, Kamidono S: Assessment of
health-related quality of life in...
... was involved in
writing the manuscript. PU was the primary researcher,
was responsible for co-ordinating and managing the study
on a day-to-day basis, for data collection and collation,
data analysis ... report.
Background
The measurement of health-related quality of life (HRQL)
is increasingly becoming part of the overall assessment of
a patient's health...