Báo cáo hóa học: " Hepatitis B virus (HBV) genotypes in Egyptian pediatric cancer patients with acute and chronic active HBV infection" ppt

... depending ona combination of the cell type and the promoter. In HEK293 cells, no significant difference in GALNSactivity was observed among the promoters used,whereas in human fibroblasts and murine ... were visualized in a 1.5% agarose gel, and band density (intensity per mm2) was measured usingimage j 1.38 x (http://rsb.info.nih.gov/ij/, National Insti-tutes of Health, USA). Band density was ... Tomatsu2 and Luis A. Barrera11 Institute for the Study of Inborn Errors of Metabolism, Pontificia Universidad Javeriana, Bogota´D.C., Colombia2 Department of Pediatrics, School of Medicine, Saint...

... cell lines werecultured in RPMI1640 medium (GIBCO, Grand Island,NY) containing 100 U/ml of penicillin, 100 mg/ml ofstreptomycin (GIBCO), and 10% heat-inactivated fetalbovine serum (FBS) (Sigma ... staining with FITC-conjugated anti-CD8 mAb and APC-conjugated anti-CD3 mAb and 7AAD (BecktonDickinson Biosciences) at 4°C for 30 min. Flow cytometric analysis was performed using FACSCalibur and ... minimal lysis)/(maximal release - minimalrelease)] ×100. Minimal lysis was obtained by i ncubat-ing the target cells with the culture medium alone, and maximal lysis was obtained by exposing...

... albumin in PBS and incu-bated overnight with monoclonal antibody against chlamydial lipopolysaccharide (NearMedic Plus, RF). After washing in PBS sections were incubated for 2 hours with goat ... contrasting with 2% uranyl acetate. Acetone-dehydrated speci-mens were embedded into LR White Resin for ultra-thin sectioning. The sections were blocked for 1 hour with 0.5% bovine serum albumin ... (C and D). Figure 4. Electron-microscopic images. Immunogold labeling of C.pneumoniae elementary bodies in serum sediments. A – preincubation with normal mouse IgG. B – preincubation with...

... medications are becoming more and more necessary in our patients with non-alcoholic steatohepatitis and certainly in our patients with HCV and metabolic steatosis. 3. HCV-Induced Steatosis The ... be found in patients infected with hepatitis < /b> C virus (HCV). Metabolic steatosis can coexist with HCV, regardless of genotype, in patients with risk factors such as obesity, hyperlipidemia, and ... AM, Al Mardini H, Awad S, et al. Quantitative assessment of fibrosis and steatosis in liver biopsies from patients with chronic hepatitis < /b> C. J Clin Pathol 2001;54:461-5. 23. Rubbia-Brandt L,...