... functions of AICAR, we tested its inhibitorypotential in blocking the expression of pro-inflammatory cytokines and iNOS, expression of COX-2, generation of ROS, and associated signaling following ... cytokine, iNOS, COX-2 and MnSOD genes, and by inhibition of ROS generation and depletion of glutathione in astroglial cells. AICAR also inhibited down-stream signaling leadingto the regulation of ... (1–42) in concentrations as indicated. The corresponding reverse peptide (40–1) in lane 3 and 9 served as a positive control in this assay. The production of NO (top) and expression of iNOS,...