... (QIAGEN, Ger-many), the quality of RNA was analyzed in 0.8% agarosegel stained with ethidium bromide and the concentrationwas determined by spectrophotometric analysis at 260and 280 nm (Lambda ... DC, McFarland RD, Keiser PH, Gage EA, Massey JM, Haynes BF, Polis MA, Haase AT, Feinberg MB, Sullivan JL, Jamieson BD, Zack JA, Picker LJ, Koup RA: Changes in thymic function with age and during ... findingssuggest that an impaired thymic output function and, as a consequence, an altered ability to maintain T cell homeo-stasis, which may play an important role in the immuno-deficiency in CML patients. ...