báo cáo hóa học: " Soluble HLA measurement in saliva and cerebrospinal fluid in Caucasian patients with multiple sclerosis: a preliminary study" pdf

... measurement in saliva and cerebrospinal fluid in Caucasian patients with multiple sclerosis: a preliminary studyIrena Adamashvili1, Alireza Minagar1, Eduardo Gonzalez-Toledo2, Liubov Featherston1 ... phosphate pooled and dialyzedagainst saline. Total protein was quantitated with thePierce BCA kit® (Rockford, IL), which was assumed to bepure sHLA-I. With each assay, a standard curve was ... in autoimmune disorders.Methods: We applied a solid phase, enzyme-linked immunoassay to measure soluble HLA class I(sHLA-I) and class II (sHLA-II) molecules in the saliva and cerebrospinal fluid...

báo cáo hóa học: " Saliva soluble HLA as a potential marker of response to interferon-β1a in multiple sclerosis: A preliminary study" pdf

... Gonzales-Toledo E, Featherston L, Kelley R: Soluble HLA measurement in saliva and cerebrospinal fluid in Caucasian patients with multiple sclerosis: a preliminary study. J Neuroinflammation 2005, 2:13-16.17. ... (Fig-ures 1A and 1B). All study subjects with RRMS hadmeasurable amounts of sHLA-II in their saliva and in allsubjects increases in saliva sHLA-II levels following treat-ment with IFN-β 1a (at month ... solid phase ELISA was used to quantitate s -HLA- I and s- HLA- II in the saliva obtained from study subjects. Theimmuno-affinity purification and quantitation of s -HLA- I and s -HLA- II has been previously...

Báo cáo hóa học: "Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients" ppt

... generated during the rear-rangement of IGK chain that remain stably present in the genome and are duplicated during each cell division.Statistical analysisSince data did not follow a Gaussian distribution, ... DiscussionCharacterization of CLL patients All patients enrolled in this study were in a very earlystage of disease (Rai stage 0, Binet stage A) and had notbeen previously treated. Their demographic and labora-tory ... parameters are shown in Table 1. The analysis ofbiological prognostic factors showed 7 (58%) patients with mutated IgVH, 6 (50%) patients with 13q14deletion at FISH analysis, and 3 (25%) patients...

Báo cáo hóa học: "The relation between neuromechanical parameters and Ashworth score in stroke patients" ppt

... norma lized parameter covariance matrix forall model parameters is visualized in Figure 6 (top). Onthe average, the auto-covariance (diagonal) was largerthan the cross-covariance (off-diagonal) ... controls and thestroke patients with an AS of one and higher. Increasedstiffness was not s ignificantly higher for patients with AS0 compared to controls, indicating small differences with a statistical ... damage,such as stroke and may interfere with function. Itsassessment and treatment are therefore major goals in rehabilitation. Main contributors to increased joint resis-tance are increased viscosit...

báo cáo hóa học: "Kinematic aspects of trunk motion and gender effect in normal adults" pdf

... understanding. The kinematic and basic gait data such as walking speed, cadence, and stride length were obtained separately for the right and left sides, and overall 40 sets of data were included ... Kakinoki R, Kwanabe K, Kanzaki H, Mito Y,Nakaura T: Gait analysis of patients in early stages after total hiparthroplasty: effect of lateral trunk displacement on walking efficiency.Journal ... England) captured marker movements at a sam-pling rate of 60 Hz, and three trials were averaged to a single data set. For each trial (9-meter walk) one gaitcycle, which was not in the initial...

báo cáo hóa học: " Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene" ppt

... brainstem, and rednucleus of the midbrain. Other brain regions, including substantia nigra, cranial nerve nuclei,hippocampus and cortex showed normal appearing microglia. In animals during end stage ... purposes)Visualization of microglia in midbrain with GSA-I-B4 lectin (A- F) and in motor cortex with OX-42 (G) and OX-6 (H) during symptomatic diseaseFigure 5Visualization of microglia in midbrain with GSA-I-B4 ... anesthetized and transcardially per-fused with phosphate buffer saline (PBS) followed by a fixative solution containing 4% paraformaldehyde. Thespinal cord and brain were dissected out and fixed 2 h in 4%...

báo cáo hóa học:" Alendronate increases BMD at appendicular and axial skeletons in patients with established osteoporosis" docx

... commercial available ELISA kitPYRILINKS-D (Metra Biosystem, USA) [8,13]. SerumBALP and urinary DPD were measured at both baseline and follow-up at 6 and 12 months.Dropout and facture caseBoth ... 0.01.Table 2: BMD data at baseline, 6- and 12-months. Comparison of BMD at baseline and its changes at 6- and 12-months in patients with established osteoporosis compared between Alendronate group ... phos-phatase (BALP) and urinary Deoxypyridoline (DPD) in both Alendronate group and control group at baseline and their changes at 6-months and 12-months. The per-centage difference is also shown in...

báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx

... grants NAG-2-1286, NCC2-1361 and the Department ofVeterans Affairs Medical Research Service in support of MHF and this project.We thank Sandra Spurlock for the data plate reading and data analysis ... neutralized with 10%ammonium hydroxide and the absorbance of AlizarinRed was measured at 450 nm using a microplate reader.Data is expressed i n absolute amounts accor ding to a standard curve.RNA ... the absorbance of AlizarinRed was measured at 450 nm using a microplate reader (n = 6). Data isexpressed at in absolute amounts according to a standard curve. 5%NormalMedia...

báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf

... was neutralized with 10%ammonium hydroxide and the absorbance of AlizarinRed was measured at 450 nm using a microplate reader.Data is expressed i n absolute amounts accor ding to a standard ... NASA grants NAG-2-1286, NCC2-1361 and the Department ofVeterans Affairs Medical Research Service in support of MHF and this project.We thank Sandra Spurlock for the data plate reading and data ... per microgram protein.Alizarin Red visualization of mineralizationAlizarin Red (2%) stained cells were incubated with 10%acetic acid for 30 minutes to release bound Alizarin Redinto solution....

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