... o f potassium phosphate between < 0.05 and 80 mM. PLP at a concentration of between 0.0 and 100 lMwas added to reconstitute the holo-enzyme. Thereaction was carried out at 30 °C and typically, ... citrate and 0.1Mcysteine hydrochloride, in short, the resolution buffer.Various pH values between 5.0 and 8.0 were tested, and apH of 7.0 was chosen (see below). Prior to the resolution,CcStP and ... Exceptfor R226A and R242A mutants (Table 1), all enzymes werestable for 2 h in the presence of 5 mMpotassium phosphate and potassium sulphate.Reconstitutions with PLP of apo-CcStP and mutantsthereof,...