... protein loading betweensamples, actin protein in each sample was detected byWestern blotting using an anti-actin antibody (Actin(1–19)-HRP, Santa Cruz, 1:2000). In addition, proteinloading ... RealTime PCR System using the following cycling parameters: Step 1: 50°C, 2 minutes; Step 2: 95°C, 10 minutes; Step 3: 95°C, 15 seconds; Step 4: 60°C, 1 minute; repeat Steps3 and 4, 39 more ... 7500Real Time PCR System using the following cycling param-eters: Step 1: 50°C, 2 minutes; Step 2: 95°C, 10 minutes; Step 3: 95°C, 15 seconds; Step 4: 60°C, 1 minute; repeatSteps 3 and 4, 39 more...