Báo cáo sinh học: "Mutational analysis of the potential catalytic residues of the VV G1L metalloproteinase" pptx
Báo cáo sinh học: "Mutational analysis of the potential catalytic residues of the VV G1L metalloproteinase" pptx
... byimmunoblot.Mutational analysis of the putative catalytic and zinc-binding residues of G1L utilizing a trans complementation assayFigure 4Mutational analysis of the putative catalytic and zinc-binding residues of ... to wild type G1L and is the culmination of two independent experiments. With the exception of wild type G1L, the E120A mutant and, to alesser extent the E35A mutant, none of the other mutantswere ... (TET)-dependentrecombinant VV, vvtetO :G1L. The construction of vvtetO :G1L is described in detail in 27. Briefly, the TEToperator was inserted directly upstream of the G1L openreading frame. When...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... complex,cleavage of the P1–P1¢ peptide bond, linkage of the activesite Ser of the protease to the carboxyl group of P1by anester bond and insertion of the N-terminal part of the RCLas strand ... cognateproteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing structural rearrangements of the serpin stabilizes the molecule in a so-called ... loss of activitysuggesting a heterogeneity in the active fraction of thesevariants. That substituting either of the juxtaposed residues E283 (s6A) or K327 (s5A) with an A increases the rate of latency...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... indica-ted by the numbers on the right side. The migration of the proteinmarkers is indicated by pencil marks on the membrane. The arrowindicates the position of the wild-type and N130D variants of human ... corrected by subtracting the spectrum of the buffer solution (5 mm Tris ⁄ HCl, pH 7.5) in the absence of protein. The stability to thermal inactivation was examined byincubation of the enzymes at 80 ... [25]. From these studies, the a-carboxylategroup of arginine appears to be hydrogen bonded to the N130 carboxamide NH2group and the S137hydroxyl group of arginase I [9,23,24]. These residues and...
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Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
... a catalytic triade. No furtherefforts were made to track down the actual target of the inhibitors.DISCUSSION The purpose of t his work was to identify the key residues in the C-domain a nd their ... Amilestone in the study of peptide s ynthetases was the determination of the A-domain structure of GrsAPhe-A [8].Aspects of speci®city and binding were superimposed onother A-domains and with the help ... avor of the latter mode of action, and we a lso were not able to detect a conservedserine residue that might act in the catalytic center of C-domains. To further rule out the possibility of a catalytic triade,...
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Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt
... Three of them (CRB-3–5) are in the vicinity of two putative coiled-coil domains, whichsuggests that they may participate together with the coiled-coil domains in folding of the N-terminal half of ... domains may positionthem in a way that they can contribute to the forma-tion of higher order structures of the Mrs2p part on the inner side of the membrane, which may contributeto the proposed opening ... located in a block of conserved amino acid residues at the start of the secondcoiled-coil domain (CC2), which is conserved among the Mrs2-CorA protein family (Fig. 1). Analysis of thiscoiled-coil...
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Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot
... datanot shown). On the basis of these results, we concludethat the absence of either of these two clusters of basicamino acid within the linker does not affect the ability of the protein to preferentially ... tyrosine on the scissile phosphate in the DNA, resulting in the cova-lent attachment of the enzyme to the 3¢ end of the broken strand [2]. Rotation of the duplex regiondownstream of the break ... manifestation of the preferencefor supercoils. It is noteworthy that the elimination of either of the clusters of basic amino acids within the linker region (Fig. 1) does not affect the preference of the...
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báo cáo sinh học:" Using nurses to identify HAART eligible patients in the Republic of Mozambique: results of a time series analysis" potx
... site.Outcome 3 The final analysis determined whether the introduction of the CD4 nurse increased the monthly number of adultpatients starting HAART. The outcome was the number of eligible patients ... including the study site, the study month (a meas-ure of the effect of time), the number of monthly MD/MOvisits (a measure of MD/MO capacity), and the number of monthly new enrollees at the clinic ... efficiencywere a source of improvements in outcomes over the study period. These time trends may have obscured the potential impact of the CD4 nurse intervention, and the effect of the intervention...
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Báo cáo sinh học: " Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" potx
... andincrease in diversity of the T cell repertoire was detectedin the pancreases, with most of the BV families presentat pre-diabetic and d iabetic stages. A similar expansionprofile of the BV TCR repertoire ... post-B cell reconstitution, the majority of the animals present pancreatic TCR expansions (at 13weeks of life). Of note, these animals do not have clono-typic expansions in their pancreatic lymph ... apolyclonal profile of induced clonotypes, with BV2, BV10, BV12 and BV14 being present on over 60% of the animals, followed next in appearanceby BV8S3 and BV11, present in 50% of the mice. B)...
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báo cáo hóa học:"Mutational analysis of the potential catalytic residues of the VV G1L metalloproteinase" docx
... byimmunoblot.Mutational analysis of the putative catalytic and zinc-binding residues of G1L utilizing a trans complementation assayFigure 4Mutational analysis of the putative catalytic and zinc-binding residues of ... conditional mutant VV infection[26]. The role of both the full-length and cleaved products of VV G1L continue to be investigated as does the ability of G1L to coordinate a zinc ion. If G1L is recognized ... 3:d354-364.BioMed CentralPage 1 of 7(page number not for citation purposes)Virology JournalOpen AccessShort reportMutational analysis of the potential catalytic residues of the VV G1L metalloproteinaseKady...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... described in the Materials and methods. Completion of the hydrolysis wasconfirmed by the disappearance of the (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The ... the conversion of glutaminyl to pyroglutamyl residues. The hydrolysis reaction was monitored by calculating the sum of the relative intensities of the peaks corresponding toGln-ONC (M23L) (theoretical ... calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthattheformation of the N-terminal pyroglutamyl is not dependenton pH in the...
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