... Repository)was Flag-tagged at the 5¢-end by PCR using the pri-mers 5¢-CTGCAGCATGGACTACAAGGACGACGATGACAAGGAGAGTTGCTACAACCCAGGTCTG-3¢and 5¢-GAGAGTTGCTACAACCCAGGTCTG-3¢with pRSV-p49 as a template. The amplified ... 5¢-AAACGTAAAAGGGCATATGAGACCTTCAAGAGCATC-3¢ for T30 5A (mutations in bold). The accuracy of the mutations wasconfirmed by DNA sequencing.p49 (obtained from the NIH AIDS Reagent Repository)was ... lymphocyte calcium signaling andage-related diseases. In Calcium Homeostasis and Signaling in Aging. Advances in Cell Aging and Gerontology,Vol.10(Mattson,M.P., ed), pp. 127–145. Elsevier, Amsterdam.23.Takuma,K.,Phuagphong,P.,Lee,E.,Mori,K.,Baba ,A. &Matsuda,...