... Anotherfunction of AclB was found to be stabilization of the enzyme, as AclB prevented the degradation of AclA thatwas otherwise observed in the absence of AclB.After the phosphorylation, ... mechanism of mammalian ACL [23], the final step of the reaction can be assumed to be the nucleophilic attack of CoA to the phosphorylated carbonyl carbon of citrylphosphate, and the cleavage of the ... 4½S3=a2bK3Sþ½S4=a3b2cK4SÞwhere v is the initial velocity of the reaction, Vmaxis the maximum velocity, [S] is the concentration of citrate, Ksis the dissociation constant of the enzyme-citrate (ES) com-plex,...