... at a pH of 6.8 in the absence of phosphatase inhibitors. For the enzymes measured in the reverse direction, the Vmaxvalues were recalculated in the direction of the flux. Toobtain these Vmaxvalues ... enzyme of interest should be investigated in the context of the in vivo-like medium. Finally, in vivo, the enzymes are present at much higher concen-trations than in typical enzyme assays, in which ... compromised the simplicity of the prepara-tion. Furthermore, some of the ions added to the assaymedium vary quite substantially in the cell as aTable 4. Vmaxvalues measured under the in vivo-like...