... the tissuesproducing it. In response to tissue injury, such ashepatic and renal injury, the inactive single-chain HGF is converted to a two-chain form exclusively inthe injured tissue. This ... C-terminus with myc-His. The enterokinase-cleaved recombi-nant pro-TMPRSS13, the disulfide-linked two-chain form, is illustrated at the bottom. (B, C) Immunoblot analysis of the recombinant pro-TMPPRSS13 ... exclusively inthe injured tissue. This conversion is mediated by ser-ine protease activity, which is induced in the injuredtissue [28]. The two-chain form is required for the bio-logical activity...