... shows a four-category data configu-
247
Development and Use of a Gold-Standard Data Set for
Subjectivity Classifications
Janyce M. Wiebet and Rebecca F. Bruce:[: and Thomas P. O'Harat
tDepartment ... reliably annotated gold standard to support
experimenting with such applications.
This research is also a case study of ana-
lyzing and improving manual tagging...
... were as follows:
forward primer, GTT GGA ATT CCA TCA TCA TCA
TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and
reverse primer, GAT CGG TAC CTC ATT ACA GAG
GAG GGA TAT GGG GAA C. The PCR reaction was
done ... T. reesei DNA
with the following primers: forward, GGG GAC AAG
TTT GTA CAA AAA AGC AGG CTA TCA TGC TGT TGT
CAG GTC CCT CTC G; and reverse, GGG GAC CAC
TTT GTA CAA GAA AGC TGG GTC A GT GG...
... requirements"
and has to do with the complexity of the task of
creating a lexical entry. It can roughly be viewed
as a measure of the time it takes to create a new
lexical entry, and of the amount of ... just a
particular set of grammatical features such as
category, gender, number, person, case, etc. A
lexeme contains all the information shared by all...
... was inhibited not only by
unlabeled Bip-Pro itself, but also by well known sub-
strates of H
+
⁄ peptide cotransporters, such as Gly-Sar,
Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,
d-aminolevulinic ... 5907
Synthesis and characterization of a new and radiolabeled
high-affinity substrate for H
+
/peptide cotransporters
Ilka Knu
¨
tter
1
, Bianka Hartrodt
2
,Ge
´
za To
´
th
3...
... (5¢-GGCCTCATGAAGAAAAAGGTCGTCA
TAATT-3¢), and TG101 (5¢-GGCCAAGCTTCTAGAAC
TTGAGAACCCTAGC-3¢) (for the P. horikoshii CoADR),
and TG104 (5¢-CGCGCCATGGAAAAGAAAAAGGTA
GTCATAA-3¢) and TG105 (5¢-CGCGGTCGACCTAGAA
CTTCAAAACCCTGGC-3¢) ... tube and
sealed with Parafilm. For lower temperatures, the tube was
placed in the respective freezer and standard activity assays
were performed after 1...
... of application domain.
• Development of a manually-bracketed corpus (tree-
bank)
of the domain.
• Creation of a grammar with a large coverage of a
blind test set of treebanked text.
Statistical ... range of sentence types and
the availability of large corpora of computer manuals. We
amassed a corpus of 40 million words, consisting of several
hundred...
... protein of Brassica, spinach, barley and
A. thaliana,PR1ofPhaseolus and MHF
9
of A. thaliana.
These proteins b elong to the 2Cys-Prx subfamily. A ll plan t
2Cys-Prx proteins, except BAS1 of barley, ... X-ray ® lm at )20 °Cor,
when using an intensifying s creen, at )80 °C.
RNA for Northern analyses was isolated as described
above and separated in a 1.3% agarose/formaldehyde...
... enzyme has a tetrameric conformation with a molecular
mass of 155 kDa. The catalytic activity of the enzyme increases up to
100 °C, and a half-life of 11 min at this temperature indicates its ... (5¢-GCGCG
GGATCCTCATTTAAGCAT
GAAAACAACTTTGCC, antisense), containing NcoI and
BamHI sites (underlined in the sequences). In order to
introduce an NcoI restriction site, an extra a...
... blot analysis of total genomic DNA from tomato
plants. Fifty micrograms of DNA was digested overnight with
restriction enzymes and separated on a 1% agarose gel. M, size
standard; lane 1, Bam HI-digested ... scions.
A strong candidate for a transmissible jasmonate signal is
the JA-conjugate, MeJA. The volatile ester can diffuse
through membranes and c an be found in the head...