... arrows).Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer-tained at this point. A cross-reaction of the antibodyagainst the 39 kDa subunit of ... piericidin are speci-fic inhibitors of complex I in eukaryotes, we assumethat they bind to the large form of the complex,which contains a homolog of the B14 subunit of mammalian complex I. The ... access the flavin cofactor in the large form of complex I,whereas the cofactor remains accessible in the smallform. Indeed, to address this possibility, experimentswith prolonged preincubation...