Báo cáo sinh học: " Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" potx
... post-
reconstitution. This allowed us to identify the < /b> expanding
TCR repertoire < /b> infiltrating the < /b> islets of < /b> NOD.< /b> Igμ
null
mice < /b> that are dependent on B cells. We observed that
without B cell < /b> reconstitution, ... capsule of < /b> diabetic < /b> B cell < /b> reconstitu ted
NOD.< /b> Igμ
null
mice < /b> were rejected very fast (within 4 d...
... post-
reconstitution. This allowed us to identify the < /b> expanding
TCR repertoire < /b> infiltrating the < /b> islets of < /b> NOD.< /b> Igμ
null
mice < /b> that are dependent on B cells. We observed that
without B cell < /b> reconstitution, ... implants were removed for
spectratyping < /b> analysis < /b> of < /b> TCR repertoire < /b> of < /b> inf iltrating T
< /b> cells...
... E120A mutant and, to a
lesser extent the < /b> E35A mutant, none of < /b> the < /b> other mutants
were capable of < /b> complimenting the < /b> conditional mutant
virus grown in < /b> the < /b> absence of < /b> TET. These results are in
< /b> accordance ... inhib-
ited G1L processing. This may be indicative of < /b> either the
< /b> loss of < /b> a zinc ion, which would render a m...
... examine the < /b> stability of < /b> the < /b> 6A3 epitope.
Epitope mapping of < /b> 6A3 may be the < /b> most direct
approach for answering the < /b> above questions [21].
In < /b> the < /b> present study, we first show that the < /b> inhibi-
tory ... solu-
ble rDPP IV lost the < /b> ability to bind to immobilized
MTF7 cell < /b> surfaces to the < /b> same degree that polyclonal...
... to be present i n the < /b> LPS
of < /b> all of < /b> these strains ( data not shown). S tructural a nalyses
have shown that PCho can be linked to terminal hexose
residues attached to either one of < /b> the < /b> three inner-core
heptose ... erely the < /b> members of < /b> the < /b> Finnish
Otitis Media Study Grou p at the < /b> National P ublic Health Institu te in
< /b> Finland...
... rate constant of < /b> the < /b> J–I
phase compared to the < /b> amplitudes and rate constants
of < /b> the < /b> two other phases. The < /b> above should be gov-
erned by the < /b> balance between the < /b> rate of < /b> reduction
and oxidation of < /b> ... demonstrating that the < /b> J–I rise
is not directly related to the < /b> reduction of < /b> the < /b> PQ pool.
A furthe...
... (one of < /b> 5¢-AAGCT
TCACCATGTACCCTGCCCACATGTACCAAGTG
TAC-3¢,5¢-AAGCTTCACCATGCCGCACCGG CTC
ATCGAGAAAAAGAG-3¢,5¢-AAGCTTCACCATG
GCAGTGGTTCTTGAACTTACCTTGAAGC-3¢ or
5¢-AAGCTTCACCATGATTGCCCTGCAGAGTGG
TTTACAAGCTG-3¢) ... region, including the < /b> PAS -B
domain of < /b> BMAL1, is required for the < /b> binding to DEC1,
Fig. 4. Identification of < /b> the < /b> domain of < /b> D EC1
that i...
... due to FRET from certain Trp residues in < /b>
the < /b> C-terminus of < /b> HpPex5p to the < /b> dansyl cap in < /b> the < /b> PTS1
peptides, the < /b> distance between the < /b> dansyl cap in < /b> L2 and the
< /b> three tryptophan residues in < /b> the < /b> model ... cytosolic ribosomes. In < /b> order to ensure that
these proteins reach the < /b> correct destination in <...
... retention time of < /b> the < /b> obtained prosthetic group
was compared with those of < /b> flavin derivatives. The
< /b> retention time of < /b> the < /b> prosthetic group bound to recom-
binant flavoredoxin was identical to that of < /b> FMN
(Fig. ... [5,6]. The < /b> crystal structure of < /b> FeR
has been determined with and without NADP
+
[6].
These authors were unsuccessful in...
... 7.
The < /b> interaction of < /b> PP2Ac with the < /b> regulatory B sub-
unit requires the < /b> extreme C-terminal region of < /b> the < /b> cat-
alytic subunit [29] and the < /b> interaction site for TIPRL
was mapped to the < /b> C-terminal ... to the < /b> C-terminus, respec-
tively.
The < /b> interaction between TIPRL and the < /b> catalytic
subunit of < /b> type 2A phosph...