... katÆmol )1 .Two mutants of yeast PGK were constructed: the W308F,W333F, Y122W triple mutant (denoted as W122), and theW308F point mutant (W333). The mutants contain a singleTrp residue either in the ... previ-ous data [39, 41] , we found that these mutants are fullyactive, thus, the mutations do not perturb the structuresignificantly. Stabilities of the mutants are also onlyslightly decreased with respect ... with the W122 and the W333 mutants,respectively.Table 1. Mid -point temperatures (T m) and calorimetric heats (Q t ) of thermal transitions of pig muscle and yeast PGKs. T m and Q t values aregiven...