Báo cáo sinh học: " Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes and monocyte-derived macrophages" docx

... for citation purposes)Virology JournalOpen AccessResearch Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes ... mutagenesis of the V3 V5 regions and binding affinity of gp120 to CCR5 and/ or gp120incorporation into chimeric virions might pinpoint themajor difference in replication efficiencies.While a co-infection ... evidence regarding the role of the HIV-1 envelope V3 V5 regions from subtype C infectedpatients from India in virus entry, coreceptor utilization and replication efficiency in primary T-lymphocytes...

... for citation purposes)Virology JournalOpen AccessResearch Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes ... biological properties of HIV-1 subtype C, including cellular tropism, virus entry, replication efficiency and cytopathiceffects, we reciprocally inserted our previously characterized envelope V3 V5 ... sequences. Thedifference in amino acid sequences in V3 to V5 regions in subtype C chimeras as compared to subtype Bsequences may be responsible for increased replication efficiencies of subtype C...

... RT-PCR using forward 5'-CCCCATTTTCCTCTAGCGACTG-3' and reverse 5'-CGGCCGCATGGT-TCTCGCCAAT-3' primers (regions corresponding to 15,386 to 15,846 bp of the PRRSV VR-2332) and ... for 30 min. To demonstrate the correlationbetween CD151 presence and susceptibility to PRRSVinfection, RT-PCR was carried out using CD151 specificprimers 5'-CCTACCTGGCCACAGCCTAC-3' ... transfection of siRNA against CD151{Fig. 6B (2)}.Blocking activity of anti-CD151 Ab on PRRSV infection into MARC-145 cells To investigate the effect of polyclonal anti-CD151 Ab onPRRSV infection...

... R5-TM-F,CATGGAGATGTTAAATTTAGATTATAAATTAGGAGTAGGAGCATTGATAGTAGCACTAATCATAGCAATAGTCGTGT-GGACCATAGTATATATAGAATAGG and R5-TM-R, AATTCCTATTCTATATATACTATGGTCCACACGACTATT-GCTATGATTAGTGCTA CTATCAATGCTCCTACTC-CTAATTTATAATCTAAATTTAACATCTC. The cytoplasmicdomains ... NL-TM-R,AATTCCTATTCTATGATTACTATGGACCACACAGCTATTGCTATTATTATTGCTACTACTAATGCTACTATTGCTAC-TATTATAGGTTGCATCTC; for the R5 vpu TM region, R5-TM-F,CATGGAGATGTTAAATTTAGATTATAAATTAGGAGTAGGAGCATTGATAGTAGCACTAATCATAGCAATAGTCGTGT-GGACCATAGTATATATAGAATAGG ... vector and sequenced.The following PCR primers (with restriction sites shown in italics) were used: for NL vpu, NL-X-F, GAATTCAT-GCAACCTATAATAGTAGCAATA and NL-R-GFP, GGATC-CGCG CAGATCATCAATATCC;...

... (5’-GTCGAC(Sal I) CACCATGGAGGCCGTG-GAGACCGG-3’ )andhNIS-3(5’-TTAATTAA(Pac I)TCAGAGGTTTGTCTCCTGCTGGTCTCGA-3’ ). ThePCR product was gel purified, and cloned into the pCR-Blunt II-TOPO vector using ... triplicate.GLV-1h153 was cytolytic against PANC-1 cells in vitro To investigate whether expression of hNIS would affectcytolytic activity of VACV in cell cultures, PANC-1 cellswere infected with GLV -1h68 ... expressed as the percentage of surviv-ing cells as compared to uninfected control. In vivo tumor therapy studies and systemic toxicityAll mice were cared for and maintained in accordancewith animal...

... negative cga gac ctc ccg ggg cac tcg caa gca cccHCV 3 negative acg cag aaa gcg tct agc cat ggc gtt agtHCV 4 negative tcc cgg ggc act cgc aag cac cct atc aggHutLA2 positive ggg ccg ggc atg aga cac ... positive cat gat gca cgc tct acg aga c HCV 10.1 positive ctg tga gga act act gtc ttc acg cagHCV 10.2 positive cac tcg caa cca ccc tat cagHCV 1 negative act gtc ttc acg cag aag cgt cta gcc atHCV ... REAGENT(Molecular Research Center, Inc. Cincinnati, OH). Theethanol washed protein pellet was solubilized into 200–500 µl of 1% SDS by incubating at 55 C for 10 minutes.Any remaining insoluble subcellular...

... attributed in part to an increase in circulating levels of cholecystokinin. Similarly, in rats fed a hy-percholesterolemic diet, ingestion of the alpha subunit of the soy 7S globulin (conglycinin) ... protein and carbohydrate sources, namely, soy protein isolate (SPI)-cornstarch, SPI-sucrose, cod protein (COD)-cornstarch, COD-sucrose, casein- (CAS)-cornstarch, CAS-sucrose. Rats fed SPI-cornstarch ... similar Int. J. Med. Sci. 2007, 4 77ducing intestinal cholesterol absorption and increas-ing fecal bile acid excretion, thereby reducing hepatic cholesterol content and enhancing removal of...

... (bp)CD163(XM_053094.2)Sense:AGCTGGGCTGTGCAGACAACGAntisense:TGAATGACCCCCGAGGATTTCAGC736CYP1A1(X00469)Sense:CTGGTTCTGGATACCCAGCTGAntisense:CCTAGGGTTGGTTACCAGG331CYP1A2(X01031)Sense:CAGTCACAACAGCCATCTTCAntisense:CCACTGCTTCTCATCATGGT302CYP2B1(XM_342078)Sense:TTGTTTGGTGCTGGGACAGAGAntisense:GGCTAGGCCCTCTCCTGCACA443CYP2E1(M20131)Sense:AAACTTCATGAAGAAATTGACAntisense:TCTCCAACACACACACGCTTTCC311TNF-a(X66539)Sense:GTAGCCCACGTCGTAGCAAAAntisense:CCCTTCTCCAGCTGGAAGAC346IL-6(NM_012589)Sense:GAAAGTCAACTCCATCTGCCAntisense:CATAGCACACTAGGTTTGCC678b-actin(BC063166)Sense:TTGTAACCAACTGGGACGATATGGAntisense:GATCTTGATCTTCATGGTGCTAG764T ... (bp)CD163(XM_053094.2)Sense:AGCTGGGCTGTGCAGACAACGAntisense:TGAATGACCCCCGAGGATTTCAGC736CYP1A1(X00469)Sense:CTGGTTCTGGATACCCAGCTGAntisense:CCTAGGGTTGGTTACCAGG331CYP1A2(X01031)Sense:CAGTCACAACAGCCATCTTCAntisense:CCACTGCTTCTCATCATGGT302CYP2B1(XM_342078)Sense:TTGTTTGGTGCTGGGACAGAGAntisense:GGCTAGGCCCTCTCCTGCACA443CYP2E1(M20131)Sense:AAACTTCATGAAGAAATTGACAntisense:TCTCCAACACACACACGCTTTCC311TNF-a(X66539)Sense:GTAGCCCACGTCGTAGCAAAAntisense:CCCTTCTCCAGCTGGAAGAC346IL-6(NM_012589)Sense:GAAAGTCAACTCCATCTGCCAntisense:CATAGCACACTAGGTTTGCC678b-actin(BC063166)Sense:TTGTAACCAACTGGGACGATATGGAntisense:GATCTTGATCTTCATGGTGCTAG764T ... (bp)CD163(XM_053094.2)Sense:AGCTGGGCTGTGCAGACAACGAntisense:TGAATGACCCCCGAGGATTTCAGC736CYP1A1(X00469)Sense:CTGGTTCTGGATACCCAGCTGAntisense:CCTAGGGTTGGTTACCAGG331CYP1A2(X01031)Sense:CAGTCACAACAGCCATCTTCAntisense:CCACTGCTTCTCATCATGGT302CYP2B1(XM_342078)Sense:TTGTTTGGTGCTGGGACAGAGAntisense:GGCTAGGCCCTCTCCTGCACA443CYP2E1(M20131)Sense:AAACTTCATGAAGAAATTGACAntisense:TCTCCAACACACACACGCTTTCC311TNF-a(X66539)Sense:GTAGCCCACGTCGTAGCAAAAntisense:CCCTTCTCCAGCTGGAAGAC346IL-6(NM_012589)Sense:GAAAGTCAACTCCATCTGCCAntisense:CATAGCACACTAGGTTTGCC678b-actin(BC063166)Sense:TTGTAACCAACTGGGACGATATGGAntisense:GATCTTGATCTTCATGGTGCTAG764T...

... (7) and the release of pro -in ammatory cytokines such as IL-8 (8).These cytokines can alter the secretion of mucus and induce changes in gastric acid secretion and homeostasis. They also attract ... Shp2. Instead, CagAPYcan inhibitSrc activity both by direct interaction of both proteinsor by binding of CagAPY to Csk, a negative regulator of Src [6,7]. Because Src is the primary kinase ... patho-genesis of H. pylori. There are two classical secretedvirulence factors present in H. pylori: the vacuolatingcytotoxin (VacA) and the CagA protein encoded bythe cytotoxin-associated gene-pathogenicity...

... oligonu-cleotides and nuclear extracts from SP1 cells. The nucleo-tide sequences of putative CERK-PPRE, including thesense 5¢-CTCTCCAGGCCACAGGCCAGAGCGG-3¢ and anti-sense 5¢-GAGAGGTCCGGTGTCCGGTCTCGCC-3¢sequences, ... Ceris incorporated, hydrolyzed to sphingosine and thenrecycled into intracellular Cer itself, and that accumu-lation of Cer contributes to the induction of apoptosis[20]. However, the signaling ... nuclear receptor acting as a ligand-inducible transcriptionfactor and has been implicated in the control of keratinocyte survival. In the mouse keratinocyte cell line SP1, serum starvation induced...