... 5¢-GGGAATTCCATATGAGAGACAATA
TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT
CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,and
PDE1(Lys321–Thr620) was amplified with primers 5¢-GGG
AATTCCATATGAAGAATGATCAATCTGGCTGCG
GCGCAC-3¢ ... causes the fatal
human sleeping sickness, as well as Nagana, a devastating
disease of domestic animals in large parts of sub-Saharan
Africa. While many aspects of trypanosome cell biolo...
... and
mammalian Na
+
channels. Accordingly, they are divided
into classical a- toxins that are highly active in mammalian
brain, a- toxins that are very active in insects and a- like
toxins that are active ... phaiodotoxin
The amino acid sequence o f the N-terminal portion of
phaiodotoxin was obtained by Edman degradation carried
out with an automatic apparatus Beckman LF 3000 Pro-
tein...
... Bro1
domain-containing protein with a thioester-linkage site
of isoprenoid lipid (CAAX motif, C standing for cys-
teine, A for generally aliphatic amino acid, and X for
any amino acid)] in this article, ... University, Japan
Alix (also named AIP1) is an interacting partner of the
penta-EF-hand Ca
2+
-binding protein, ALG-2 [1–5],
and acts as a multifunctional adaptor protein in vari...
... Kawazu, S., Tsuji, N., Hatabu, T., Kawai, S., Matsumoto, Y. &
Kano, S. (2000) Molecular cloning and characterization of a
peroxiredoxin from the human malaria parasite Plasmodium fal-
ciparum. ... tertian
malaria in man.
Interestingly, two similar sequence annotations (Gen-
Bank accesson numbers, NP_473166 and CAB38989) were
available that proposed a large protein of 2417 and 2396
a...
... the N-terminal fragment
of the poneratoxin gene. Two others: forward 5¢-GCC
GCCCGTGATACAGGCGATCCACGATGCGCAGA
GGTAGTAATGAG-3¢ and reverse 5¢-AATTCTCATTA
CTACCTCTGCGCATCGTGGATCGCCTGTATCAC
GGG-3¢ ... construction of
the poneratoxin gene [11]. Two oligonucleotides: forward
5¢-
2
GATCCATGTTTCTTCCGCTTCTGATCCTTGGCT
CTCTTCTGATGAC-3¢ and reverse 5¢-CGGCGTCATCA
GAAGAGAGCCAAGGATCAGAAGCGGAAGAAA
CATG-3¢,...
... ATTCCCGGGTAATTTTTCCAAGTTAAATTACTTG SmaI
rFnBPB
163–308
F GGGGGATCCGGTACAGATGTAACAAATAAAG BamHI
rFnBPB
163–308
R CTCCCCGGGCTATTGAATATTAAATATTTTGCTAA SmaI
rFnBPB
309–480
F CCCGGATCCTATTTAGGTGGAGTTAGAGATAAT ... (5¢–3¢)
a, b
5¢ restriction site
rFnBPB
37–480
F CGGGGATCCGCATCGGAACAAAACAATAC BamHI
rFnBPB
37–480
R AATCCCGGGTTACTTTAGTTTATCTTTGCCG SmaI
rFnBPB
163–463
F GGGGGATCCGGTACAGATGTAACAAATAAAG...
... oligo-
saccharides; h,LM4;
,LM5;j,LM6;s,Manb1fi2Mana1fi
2Mana1fi2Mana1fi2Man; d,Manb1fi2Manb1fi2Mana1fi2Man
a1 fi2Mana1fi2Man; n,Manb1fi2Mana1fi3Mana1fi2Mana1fi
2Man; m,Manb1fi2Manb1fi2Mana1fi3Mana1fi2Mana1fi
2Man.
2572 ... oligosaccha-
rides, Manb1fi2Mana1fi3Mana1fi2Mana1fi2Man and
Manb1fi2Manb1fi2Mana1fi3Mana1fi2Mana1fi2Man
were prepared from the mannan of C. guilliermondii [17]
or C. saitoana [35] and Manb1fi2Mana1fi...
... were incubated with the indicated concentrations of ACTH. In six separate experiments, the mean FFA concentration measured in
the incubation media in absence of any pharmacological agents (basal ... that acute B [a] P treatment profoundly
impaired catecholamine-induced lipolysis in both
murine and human adipocytes. Furthermore, a signifi-
cant weight gain, as well as increased fat ma...