... with grinding. 20 μlofProteinaseK(10 mg/ml) was added to the sample mixture and mixedbyvortexing.Thesamplewaslysedbyincubationat60°C for 30 minutes, and the tube was inverted every5 minutes. ... details1Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.2Keenlink Dental Clinic, Hong Kong, China.3State Key Laboratory for OralDiseases and Department of Prosthodontics, West China ... hypermethylationwithin the CpG clusters, or ‘ islands’ , in the promoterregion of genes that isn’t normally methylated [1]. Thisaberrant methylation in cells causes the silencing of cer-tain genes, including...