báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... citation purposes)Journal of Translational MedicineOpen AccessResearch Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturationPhilippe ... con-centration of 2–5 × 106 of cells per 200–500 μl of medium. After 3 hours at 37°C, cells were placed in com-plete medium and analysed by flow cytometry betweenday 5 and day 60. Transduction of CD34-pDCSemi-adherent ... Trono D, Kin-dler V: Transduction of CD34+ cells with lentiviral vectorsenables the production of large quantities of transgene-expressing immature and mature dendritic cells. J Gene Med2001,...
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báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
... presence of CD3+ and CD45+ cells was usedas an indicator of T cell infiltration and the prevalence of CD4+, CD8+, CD56+ cells as indicators of T helper, T cyto-toxic and NK cells, respectively ... mononuclear cells (PBMNC). After 6 infusions and a pause of 3months, the patient received another 3 cell infusions. The phenotypic characteristics and activation status of tumor and effector cells ... infiltrating T and NK cells. In the primary tumor and in the anastomotic relapse there was a strong infiltration of CD3+/CD4+ T cells, but no infiltration of antigen pre-senting cells, as determined...
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báo cáo hóa học:" yuDetecting the percent of peripheral blood mononuclear cells displaying p-STAT-3 in malignant glioma patients" pot
... wasdone using 5 μL of FITC labeled antihuman CD4(Pharmingen, San Diego, CA) in 45 μL of FACS buffer and 5 μL of APC-labeled antihuman CD25 (Pharmingen) for15 min at 4°C. Cells were then washed ... (MHC)II, CD80, CD86, and IL-12 in dendritic cells renderingthem unable to stimulate T cells and generate antitumorimmunity [15] and enhancing Treg activity [20]. Withinthe immune cells, γ-IFN has ... FOXP3expression in human CD4+CD25+ regulatory T cells througha STAT-dependent mechanism and induces the expansion of these cells in vivo. Blood 2006, 108:1571-1579.21. Kortylewski M, Yu H: Role of Stat3...
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Báo cáo hóa học: " Single-trial classification of motor imagery differing in task complexity: a functional near-infrared spectroscopy study" ppt
... Zurich,Frauenklinikstrasse 10, 8091 Zurich, SwitzerlandFull list of author information is available at the end of the articleHolper and Wolf Journal of NeuroEngineering and Rehabilitation 2011, 8:34http://www.jneuroengrehab.com/content/8/1/34JNERJOURNAL ... combina-tions of mental tasks, signal features and classifiers.Sitaram et al. [4] performed offline classification of handmotor imagery (MI) using mean amplitude changes inΔ[O2Hb] and Δ[HHb] ... corresponding to ‘yes’ and ‘no’ in response to aseries of questions. An average offline classificationaccuracy of 80% was achieved in 40% of the locked-inpart icipa nts using maximum and mean Δ[O2Hb]...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells. Stem Cells. 2004;22(5):779-89. 10. Speicher MR, Ballard SG, Ward DC. Karyotyping human ... use of temperature-heated stage and pre-warmed and pre-equilibrated culture media. Nevertheless, the results of this study would prove otherwise. hESC of the H9 line isolated by Thomson and ... 2006.07.25 This study investigated the effects of exposing human embryonic stem cells (hESC) to 4oC and 25oC for extended durations of 24h and 48h respectively. Cell survivability after...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... mechanism of globin m-RNA stability in human RBCs. Further experiments are needed to understand the mechanisms and the biological meaning of these findings. But gene expression profiling of human ... corresponding to 8400 human genes. Here we report that human RBCs contain typical eu-karyotic RNA with 28S- and1 8S-rRNA standard bands. Microarray studies revealed the presence of transcripts of 1019 different ... total human RNA of RBCs (Bioanalyzer 2100 Agilent, USA): A) L- RNA 6000 ladder (Ambion, USA); 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of total RNA of human...
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báo cáo hóa học:" Short-term cultured, interleukin-15 differentiated dendritic cells have potent immunostimulatory properties" ppt
... Eigler A: Combined use of toll-like receptor agonists and prostaglandin E(2) in the FastDC model: rapid genera-tion of human monocyte-derived dendritic cells capable of migration and IL-12p70 production. ... In view of these data, it might be of particular interest to further elab-orate on the phenotypic and potential functional resem-blance of IFN-DCs and IL-15 DCs.While it induces full phenotypic ... (Figure 2a and 2b) and "standard"mature IL-4 DCs (Figure 2c). As shown in figure 2, matu-ration of IL-15 DCs and IL-4 DCs was associated with anupregulation of CD40, CD80, CD86 and of the...
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Báo cáo khoa học: "An Efficient Implementation of a New POP Model" doc
... probability of T is givenbyP(7) = Edrli P(tid)Thus DOP1 considers counts of subtrees of awide range of sizes in computing the probability of a tree: everything from counts of single-levelrules ... treebank. Thatis, the probability of a subtree t is taken as thenumber of occurrences of t in the training set, I t I,divided by the total number of occurrences of allsubtrees t' with ... counts of entire trees. A disadvantage of this model is that an extremely large number of subtrees (and derivations) must be taken intoaccount. Fortunately, there exists a compactPCFG-reduction of...
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báo cáo hóa học:" Anti-angiogenic effect of high doses of ascorbic acid" pptx
... exposure of cells to10–50 mg/dl of AA during 3–5 h period did not changeenergy metabolism of cells or number of cells. The level of metabolic activity was decreased on 20% for concentra-tions of ... effects of high dose AA onangiogenic activities of HUVECs (human umbilical vein endothelial cells) and HUAECs (human umbilical arterial endothelial cells) . According to our data, concentrations of ... high-concentrations of AA (post intravenousadministration of 15 g, 30 g, and 60 g) and time of expo-sure were established. The infusion of 15 g of ascorbic acidin 45 min raised the plasma level of AA to...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... differentiation and function of dendritic cells. J Immunol 2005, 174:7764-7772.45. Li L, Li SP, Min J: Hepatoma cells inhibit the differentiation and maturation of dendritic cells and increase ... expressedhigh levels of WT1 and HLA-ABC and low levels of CEAbut not HLA-DR, CD80, CD86, and CD83 molecules (Fig-ure 1B, 2A, and 5A). Fusions of DCs to the HCC cells coex-pressed the HCC cells- derived ... expression of maturation molecules of DCs/tumor fusion cells. Induction of HCC cells- specific CTL by DCs/allo-HCCTo determine whether HCC cells- reactive T cells areinduced by fusion cells, T cells...
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