nanomaterials for application in medicine and biology, 2008, p 201

... Giersig and G. B. Khomutov (eds.), 1 Nanomaterials for Application in Medicine and Biology © Springer Science + Business Media B.V. 2008 Biocompatible Nanomaterials and Nanodevices Promising for ... exciting focus for research in the bioengineering field. On the other hand, recent results obtained on nanosensory include the development of biosensing-chips for the...

... also employed for cloning large PCR fragments. The proof-reading polymerases lack 5'-3' proofreading activity and are capable of adding adenosine triphosphate residues to the 3' ... times, plasmid preps require considerable hours for cell growth and preparation of mini-prep plasmids. An additional step of screening such recombinants for the solubility of the prote...

... fusion pro- tein; inducible gene expression; ligand-binding specificity; mammalian cell lines; protein engineering; structure–activity relationship; Tet repressor; transgenic organism. Properties ... the proposal that the mutations present in rtTA [113] restrict the repressor to a noninducible conformation and lock the DNA-binding domains in the position necessary for oper- ator bindin...

... intro- duced to describe a general principle for designing genes of repetitive performance proteins for recombinant expression in Escherichia coli. In the first step, repeating amino acid sequence motifs ... therapy and tissue engineering applications (20–22). 1.2. Methodology for Creating and Studying Chimeric Proteins Between Fibritin and Triple-Stranded Segments of Fibrou...