... RNA using 100 ng random hexamer(Pharmacia, Uppsala, Sweden). The PCR primer sequencesused were as follows. FasL (forward: 5¢-ATGTTTCAGCTCTTCCACCTACAGAAGGA-3¢,reverse:5¢-CAGAGAGAGCTCAGATACGTTGAC-3¢); ... all-trans-RA concentrationsas low as 0 .1 lM, and NFAT binding was abolished in the presence of 1.0 lMall-trans-RA. In contrast, SP-1 bindingwas similar at all co ncentrations of all-trans-RA ... healthy donors bydensity gradient centrifugation of heparinized blood on a layer of Ficoll/Hypaque (Sigma, St. Louis, MO, USA). All-trans-RA, 9-cis-RA, 4b-phorbol 12-myristate 13-acetate(PMA)...