... IBO2 012
SINGAPORE
CELL & MOLECULAR BIOLOGY PRACTICAL TEST 1
23rd INTERNATIONAL BIOLOGY OLYMPIAD
8
th
– 15
th
July, 2 012
SINGAPORE
PRACTICAL TEST 1
CELL & MOLECULAR BIOLOGY
Total ... Sheet.
Q1.5 (10 points) Your skills in running a gel will be assessed by the quality of the gel
produced.
Page 7 of 9
IBO2 012
SINGAPORE
CELL & MOLECULAR BIOLO...
... miR -14 5, miR -13 3a,
miR -13 3b
Let-7 FSCN1(miR -14 5,miR -13 3a,
miR -13 3b) HMGA2 (Let-7)
(Kano et al.,
2 010 ; Liu et
al., 2 011 )
ESCC,
EAC
miR- 21 miR-375 PDCD4, NFIB, PTEN,
TPM1 (miR- 21) ;
PDK1 ... level of miR -19 6a was observed in EAC, BE and dysplastic
lesions compared with normal mucosa. MiR -14 5, miR -13 3a and miR -13 3b inhibited cell
Esophageal Cancer...
... Biotechnology
Some Eukaryotic Cell- Cycle Times
Cell Type Cell- Cycle Times
Early frog embryo cells 30 minutes
Yeast cells 1. 5-3 hours
Intestinal epithelial cells about 12 hours
Mammalian fibroblasts ... mitosis and meiosis
Lesson 1: Basics of molecular biology
I. The Cell s Organization
II. Cell Cycle and Cell Division
III. Cellular Molecules
IV. The Genetic Dogma...
... Biotechnology
Some Eukaryotic Cell- Cycle Times
Cell Type Cell- Cycle Times
Early frog embryo cells 30 minutes
Yeast cells 1. 5-3 hours
Intestinal epithelial cells about 12 hours
Mammalian fibroblasts ... mitosis and meiosis
Lesson 1: Basics of molecular biology
I. The Cell s Organization
II. Cell Cycle and Cell Division
III. Cellular Molecules
IV. The Genetic Dogma...
... cleavage of Phe
1
-|-Val
2
, His
5
-|-Leu
6
,
His
10
-|-Leu
11
, Ala
14
-|-Leu
15
, Leu
15
-|-Tyr
16
, and Tyr
16
-|-Leu
17
in the
insulin B-chain. Zn
2+
and Ca
2+
are cofactors. It is a snake venom
proteinase ... hemoglobin:
R = K
1
[p
O
2
] + 2K
1
K
2
[p
O
2
]
2
+ 3K
1
K
2
K
3
[p
O
2
]
3
+ 4K
1
K
2
K
3
K
4
[p
O
2
]
4
4 (1+ K
1
[p
O
2
] + K
1
K
2
[p
O
2
]
2...
... (Fig. 1. 1).
It is 1 3 µ (10
-4
cm = 1 µ = 10
4
Å) long and 0.75 µ in diameter. Such a
2 An Overview of Cell Structure and Function
cell contains about 2 × 10
-13
g of protein, 2 × 10
-14
g ... The Structure and Biology of Lambda 410
The Physical Structure of Lambda 410
The Genetic Structure of Lambda 411
Lysogeny and Immunity 413
Lambda’s Relatives and...