... be able to settle onto the media. 5. Holding both the lid and the tool, the right hand then flames the tool to sterilize it. 6. The red-hot tool is then inserted into the agar to cool it and ... before beginning. General References: 1. Achieving the sterile state for home tissue culture, Part I, Brian Johnson, CPS Journal 14, 18-19. 2. Achieving the sterile state for home tissue culture, ... informs me that Clonal integrity is influenced by stress factors, genotype, culture age, cutting and transfering technique, and numerous other factors still being investigated in the voluminous...