... thelatter.Heterologous synthesis of PHCP by E. coliThe E. coli System I cytochrome c biogenesis machin-ery, consisting of the Dsb and Ccm proteins, is respon-sible for the synthesis of class I cytochromes ... addition, theeffects of Dsb proteins on cytochrome c¢ synthesis have not been examined to date.In this study, we examined the heterologous synthesis of cytochrome c¢ proteins by E. coli strains ... invarious E. coli strains by means of heme-specific stain-ing of SDS ⁄ PAGE gels. On such gels, when stained materials are observed at positions coinciding withthose of PHCP and PH c552, the...