... was similar to that of the wild-type enzyme (5.7 lm) and the values of the Y48Fand H1 10F mutants were 12 times higher than that of the wild-type AKR1B3. These results confirm thatthese mutations ... and the values of the D43N,D43E, Y48F, K77R, H1 10F and H1 10A mutants were12–360 times higher than that of the wild-typeAKR1B1. However, the Kdvalue of the Y48F ⁄ H1 10Fdouble mutant of AKR1B3 ... the 9,11-endoperoxide group of PGH2to produce PGF2ain the presence of NADPH, which was defined as the PGFS activity, and the isomerization of PGH2toPGD2in the absence of NADPH or NADP+, whichwas...