... approaches and in silico analyses have resulted in several success-ful examples of systems metabolic engineering,there is still much more information embedded inlarge-scale genome-wide data and computationalsimulation ... dihydroxyacetone phosphate;gap, the glyceraldehyde 3-phosphate dehydrogenase gene; tpi, thetriosephosphate isomerase gene; dar1, the glycerol 3-phosphate dehydro-genase gene; gpp2, the glycerol 3-phosphate ... possible Several steps, reagents & enzymes requiredSpecial primers requiredSeveral purification steps involvedRID Random insertions and deletion Several steps, reagents & enzymes requiredLarge...