... volumes:http://www.springer.com/series/789615M. Amit and J. Itskovitz-Eldor, Atlas of Human Pluripotent Stem Cells: Derivation and Culturing, Stem Cell Biology and Regenerative Medicine,DOI 10.1007/97 8-1 -6 177 9-5 4 8-0 _2, © Springer Science+Business ... feasibility of feeder layer-free derivation of hESCs. A recent publi-cation by Ludwig and colleagues reported the derivation of two new hESC lines using a defi ned serum-free and animal-free medium, ... 10.1007/97 8-1 -6 177 9-5 4 8-0 _1, © Springer Science+Business Media, LLC 2012 Abstract Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass (ICM) of the developing...