... changes sensed by thefluorescence change of Trp215 takes place in DK9thrombin.Globally taken, the experimental and computationalstudies reported herein provide mechanistic support tothe phenomenological ... the unprotonated andmono-protonated thrombin form, respectively, whereas the‘s’ and p subscript refer to ES and EP thrombin species,and ‘1’ and ‘2’ superscript refer to the mono- and diproto-nated ... group of Ile16(NTIle), which holds Asp194 in a salt bridge as aresult of zymogen activation [3,5,6]. The NTIle pKaFig. 1. Analysis of pH dependence ofMichaelis–Menten constants ofD-Phe-Pip-Arg-pNA...