... 2011Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia colitransthyretin-related proteinErik Lundberg1, Anders Olofsson2, Gunilla T. Westermark3 and ... wehave investigated the fibril-forming properties of sbTTR and ecTRP in vitro, and compared the resultswith those of hTTR.Our results showed that hTTR, sbTTR and ecTRPcan form fibrillar structures, ... at 16 000 g for 30 min, after which most of the supernatant was removed and 200 lL of distilled waterwas added. The material was vortexed, and aliquots of 3–5 lL were applied to Formvar-coated...