... sites inCel74A (N213 and N417), but the purified protein wasnot extensively N-glycosylated, as treatment with endo-glycosidase H (EndoH) did not result in a reduction ofthe apparent molecular ... C-termi-nus, but also revealed that both N-glycosylation sitescarry a single GlcNAc after EndoH treatment (datanot shown). MS of the EndoH-treated Cel74A yieldeda molecular mass of 86.552 kDa. ... m)1Æcm)1calculated on the basis of the amino acidcomposition [27]. EndoH (Sigma-Aldrich, St Louis, MO,USA) treatment of Cel74A was performed according to thesupplied protocol. ESI-MS was performed with...